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货号
KVV00101
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Spike Protein has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Spike Protein Mouse IgA present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled antibody specific for Mouse IgA is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Spike Protein Mouse IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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特异性
Anti-SARS-CoV-2 Spike Protein Mouse IgA Antibody -
应用
Used for the quantitative determination of Anti-SARS-CoV-2 Spike Protein Mouse IgA concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
125 - 8,000 pg/mL -
灵敏度
47.47 pg/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (pg/mL)
3663.2
940.1
234.9
3794.4
938.8
230.5
Standard deviation
130.2
23.5
7.5
133.6
35.2
21.9
CV (%)
3.6
2.5
3.2
3.5
3.7
9.5
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
