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货号
KAD10801
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描述
This assay employs the quantitative sandwich enzyme immunoassay technique. Mouse FMC63 scFv has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-FMC63 scFv Antibody will be captured by immobilized Mouse FMC63 scFv. After washing away any unbound substances, a biotin-labeled Mouse FMC63 scFv is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-FMC63 scFv Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
1.56 - 100 ng/mL -
灵敏度
1.04 ng/mL -
精准度
CV<15% -
回收率
80-120% -
稳定性和存储
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. -
别名
Anti-FMC63 scFv antibody, CD19, CAR-T, Mouse FMC63 scFv
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背景
FMC63 is a mouse IgG2a monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. Anti-FMC63 scFv antibody can specifically bind to the antigen recognition epitope of FMC63 scFv on anti-CD19 CAR, and it shows high specificity and sensitivity, it's used to detect the expression of FMC63 scFv derived CAR.
