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货号
KAC09601
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Trastuzumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Trastuzumab will be captured by immobilized Trastuzumab. After washing away any unbound substances, a biotin-labeled Trastuzumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Trastuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
3.13 - 200 ng/mL -
灵敏度
2.94 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
92.9
25.0
10.2
89.4
23.1
10.5
Standard deviation
4.2
1.4
0.8
8.8
1.9
0.9
CV (%)
4.5
5.6
7.9
9.9
8.3
8.2
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
timigutuzumab, 4D5-8, 4D5V8, Herceptin, rhuMab HER2,CAS: 180288-69-1
