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货号
KAC90702
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Obinutuzumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Obinutuzumab will be captured by immobilized Obinutuzumab. After washing away any unbound substances, a biotin-labeled Obinutuzumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Obinutuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Obinutuzumab concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
6.25 - 400 ng/mL -
灵敏度
2.29 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
194.0
46.4
11.3
191.2
47.0
9.8
Standard deviation
8.1
2.2
1.1
7.7
4.1
1.2
CV (%)
4.2
4.7
10.0
4.0
8.7
12.0
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
GA101,RG7159,RO5072759,Afutuzumab,CAS: 949142-50-1
