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货号
KAD12652
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Bevacizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human VEGFA antigen and then pipetted into the wells. Anti-Bevacizumab Neutralizing Antibody in the sample competitively binds to Bevacizumab with the biotin-labeled Human VEGFA antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Bevacizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Bevacizumab Neutralizing Antibody concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
61.73 - 5,000 ng/mL -
灵敏度
8.26 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
2517.8
898.7
141.2
2816.2
870.1
167.2
Standard deviation
318.1
92.2
14.5
483.0
92.8
28.8
CV (%)
12.6
10.3
10.3
17.2
10.7
17.2
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
12-IgG1,F(ab)-12 IgG1,Fab-12 IgG1,rhuMAb-VEGF, ABP 215, CAS: 216974-75-3
