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货号
KAD17201
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Ipilimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Ipilimumab will be captured by immobilized Ipilimumab. After washing away any unbound substances, a biotin-labeled Ipilimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Ipilimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Ipilimumab concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
7.81 - 500 ng/mL -
灵敏度
4.25 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
223.4
60.7
11.8
225.5
49.1
12.8
Standard deviation
14.0
3.3
1.1
14.9
2.8
1.0
CV (%)
6.3
5.5
9.0
6.6
5.7
8.2
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
BMS-734016,MDX-010,MDX-CTLA-4,CAS: 477202-00-9
