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货号
KAD62601
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Dupilumab will be captured by immobilized Dupilumab. After washing away any unbound substances, a biotin-labeled Dupilumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Dupilumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Dupilumab concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
3.125 - 100 ng/mL -
灵敏度
1.32ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
59.4
28.7
5.2
63.5
28.9
7.4
Standard deviation
5.5
1.9
0.5
9.4
2.7
1.0
CV (%)
9.3
6.7
8.9
14.7
9.4
12.9
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃. -
别名
CD124,REGN668,SAR231893, CAS: 1190264-60-8
