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货号
KAD62651
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD124 and then pipetted into the wells. Anti-Dupilumab Neutralizing Antibody in the sample competitively binds to Dupilumab with the biotin-labeled Human CD124. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Dupilumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Dupilumab Neutralizing Antibody concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
46.88 - 3,000 ng/mL -
灵敏度
29.02 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
2112.5
445.2
97.2
2653.5
475.4
96.6
Standard deviation
259.1
34.9
11.1
497.1
57.4
18.0
CV (%)
12.3
7.8
11.4
18.7
12.1
18.6
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
CD124,REGN668,SAR231893, CAS: 1190264-60-8
