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货号
KVV99903
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. SARS-CoV-2 Spike has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Spike Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Goat Anti-Mouse IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 Spike Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-SARS-CoV-2 Spike Mouse IgG concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative (Competitive) -
检测范围
50 - 3,200 pg/mL -
灵敏度
25.23 pg/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (pg/mL)
1463.2
410.2
186.0
1410.7
372.1
110.1
Standard deviation
84.9
17.6
6.3
68.4
16.9
11.3
CV (%)
5.8
4.3
3.4
4.9
4.6
10.3
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
Spike glycoprotein, S glycoprotein
