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货号
KAV46401
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. CHIKV NSP1 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-CHIKV NSP1 hIgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP conjugated probe is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-CHIKV NSP1 hIgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-CHIKV NSP1 hIgG concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
0.78 - 50 ng/mL -
灵敏度
0.29 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
23.4
5.7
1.6
23.4
6.2
1.6
Standard deviation
1.5
0.3
0.1
1.1
0.6
0.1
CV (%)
6.3
5.9
7.1
4.9
9.5
7.2
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
Anti-CHIKV NSP1 hIgG, CHIKV, NSP1, NS1, hIgG, Chikungunya virus
