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货号
KAV46402
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant CHIKV NSP1 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-CHIKV NSP1 hIgM present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgM antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-CHIKV NSP1 hIgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-CHIKV NSP1 hIgM concentration in Human serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
156.25 - 10,000 ng/mL -
灵敏度
102.56 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
4339.3
1087.5
732.1
4101.1
1038.0
519.3
Standard deviation
313.6
101.8
69.8
258.5
98.6
76.4
CV (%)
7.2
9.4
9.5
6.3
9.5
14.7
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. -
别名
Anti-CHIKV NSP1 hIgM, CHIKV, NSP1, NS1, hIgM, Chikungunya virus
