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货号
KAJ53401
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Burosumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Burosumab will be captured by immobilized Burosumab. After washing away any unbound substances, a biotin-labeled Burosumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Burosumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Burosumab concentration in serum and plasma. -
样本类型
Plasma, Serum -
检测范围
15.63 - 1,000 ng/mL -
灵敏度
11.24 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
