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货号
KAB94001
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Human Insulin has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Human Anti-Insulin Antibody will be captured by immobilized Human Insulin. After washing away any unbound substances, a biotin-labeled Human Insulin is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-Insulin Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Human Anti-Insulin Antibody concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative (Sandwich) -
检测范围
3.13 - 200 ng/mL -
灵敏度
2.13 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
